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Below is the document structure we use for batch Certificates of Analysis — including HPLC chromatogram and mass-spectrometry charts. It is illustrative only; send us your order reference to request the issued COA for a fulfilled batch.

Sample — not a live batch document

www.edinburghpeptides.co.uk

Edinburgh Peptides

Document: COA-ERL-BPC157TB-2026

CERTIFICATE OF ANALYSIS

BPC157 + TB500 BlendBatch ERL-BPC157TB-202605 May 2026

Amino acid sequence

Blend — BPC-157: GEPPPGKPADDAGLV + TB-500 (Tβ4): Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES

15 + 43 (blend) residues

Analytical results

HPLC chromatogram

RP-HPLC C18 — UV detection at 220 nm — gradient 10–70% ACN in 0.1% TFA over 30 min — flow 1.0 mL/min

Main peak RT 15.67 min · Area 99.49% · Largest imp 0.32% · Total related 0.51%

Mass spectrum ESI-MS positive

Electrospray ionisation — positive mode

Observed [M+H]+ 1420.6 (BPC-157) · 848.0 (TB-500) · MW 1419.56 + 846.97 g/mol

Overall result: Conforms to specification

Acetate salt (report)

HPLC chromatogram RP-HPLC C18

020.040.060.080.01000510.015.020.025.030.0Retention time (min)Absorbance (mAU)

Mass spectrum ESI-MS positive

020.040.060.080.050010001500m/zRelative intensity (%)
Product nameBPC157 + TB500 Blend
Molecular formulaBlend (BPC-157 + TB-500)
Batch numberERL-BPC157TB-2026
Molecular weight1419.56 + 846.97 g/mol
CAS number137525-51-0 / 885340-08-9
Quantity5+5 mg
Date of manufacture05 May 2026
PubChem CID9941957 / 10169788
StoragePowder: −20°C 3 years; 4°C 2 years; 15°C 3 months.
StorageIn solvent: −80°C 6 months; −20°C 1 month; 10°C 1 week
Test parameterMethodSpecificationResultStatus
AppearanceVisualWhite to off-white powderConformsPass
Peptide purityRP-HPLC (C18)≥ 98.0%99.49%Pass
Molecular weight confirmationESI-MSWithin ± 1.0 Da of theoryConformsPass
Water contentKarl Fischer≤ 10.0%5.2%Pass
Counter-ion contentHPLCReport6.3% (acetate)Report
Total related substancesRP-HPLC≤ 2.0%0.51%Pass
Largest single impurityRP-HPLC≤ 1.0%0.32%Pass
Residual solvent — AcetonitrileGC-HS≤ 410 ppm< 410 ppmPass
Residual solvent — DCMGC-HS≤ 600 ppm< 600 ppmPass
Residual solvent — DMFGC-HS≤ 880 ppm< 880 ppmPass
Bacterial endotoxinsLAL kinetic turbidimetric< 5 EU/mg< 0.5 EU/mgPass
BioburdenUSP <61>< 100 CFU/g (USP <61>)< 10 CFU/gPass

Results relate only to the batch tested, by the stated methods at the time of release. Storage figures are recommendations, not stability data. Research & Development purposes only — not for human or animal consumption.

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